Diagnostic Genetic Sciences (DGS)

Program Director: Judy Brown

Program Office: Room 220, Koons Hall

For major requirements, see the College of Agriculture, Health and Natural Resources, Department of Allied Health Sciences section of this Catalog.

3222. Medical Cytogenetics

Four credits. Two 2-hour lectures. Prerequisite: MCB 2400 or 2410 or 2413, which may be concurrent; open to students in the Diagnostic Genetic Sciences Program; others who have met the prerequisites.

Study of human chromosomes for prenatal and post-natal detection of chromosome abnormalities, chromosome polymorphisms, cell culture and harvest, human chromosome nomenclature, staining and banding techniques, mechanisms of numerical and structural chromosome abnormality, numerical syndromes, duplication and deletion syndromes, the sex chromosomes, sex chromosome abnormalities, mosaicism, genetic imprinting, indications for chromosomal analysis, molecular cytogenetic testing.

3223. Laboratory in Cytogenetics

Three credits. One 3-hour laboratory period and two 1½-hour discussions. Four additional laboratory sessions are required during the first half of the semester. Prerequisite: DGS 3222 which may be taken concurrently; open only to students enrolled in the Diagnostic Genetic Sciences Program; others with consent of instructor.

Human chromosome morphology and identification, aseptic technique, lymphocyte culture and harvest, chromosome banding, karyotyping and microscopic analysis of normal and abnormal cases. A fee of $50 is charged for this course.

3225. Microscopy and Chromosome Imaging

One credit. Prerequisite: DGS 3223, which may be taken concurrently; open only to students enrolled in the Diagnostic Genetic Sciences Program; others with consent of instructor.

Theory and techniques of brightfield and fluorescence microscopy, and computerized chromosome imaging.

3226. Current Genetic Research

Variable credits. Prerequisite: DGS 3222 or MCB 2410 or MCB 2413; instructor consent. May be repeated one time for credit.

Retrieval, review and discussion of current primary genetics literature in addition to attending and reviewing University research seminars/guest speakers.

3999. Independent Study for Undergraduates

Credits and hours by arrangement. Prerequisite: Open only with consent of instructor. May be repeated for credit.

This course is designed primarily for students who wish to extend their knowledge in some specialized area in the field of diagnostic genetic sciences.

4095. Special Topics

Credits and hours by arrangement. Prerequisite: Open only with consent of instructor. May be repeated for credit.

Application of the scientific method of inquiry to planning, implementation, evaluating and reporting a study of a problem in cytogenetics.

4224. Cancer Cytogenetics

Four credits. Prerequisite: DGS 3222; open to juniors or higher.

Genetic basis of cancer, chromosome instability syndromes, processing of tumor samples, chromosomal changes of solid tumors and hematologic malignancies, and nomenclature of acquired changes.

4234. Diagnostic Molecular Technologies

Three credits. Prerequisite: MCB 2400 or 2410 (DGS majors must take MCB 2410) and AH 3121 or MCB 4211 which may be taken concurrently; open only to students enrolled in the Diagnostic Genetic Sciences and Medical Laboratory Sciences Programs; others with instructor consent.

DNA and RNA diagnostic technologies used in clinical settings; clinical applications in prenatal diagnosis; cancer management, transplantation, paternity testing, forensic medicine and microbiology.

4234W. Diagnostic Molecular Technologies

Prerequisite: MCB 2400 or 2410 (DGS majors must take MCB 2410) and AH 3121 or MCB 4211 which may be taken concurrently; ENGL 1010 or 1011 or 2011; open only to students enrolled in the Diagnostic Genetic Sciences and Medical Laboratory Sciences Programs; others with instructor consent.

4235. Laboratory in Molecular Diagnostics

Two credits. Prerequisite: DGS 4234 and 4236 which may be taken concurrently; open only to students enrolled in the Diagnostic Genetic Sciences and Medical Laboratory Sciences Program, others with instructor consent.

DNA isolation, blotting techniques, fluorescent in situ hybridization, polymerase chain reaction and Genprobe assay. A fee of $50 is charged for this course.

4236. Case Studies in Molecular Pathology

One credit. One 1-hour discussion. Prerequisite: Open to DGS students. Corequisite: DGS 4235.

Clinical cases in molecular pathology are presented and discussed.

4246. Contemporary Issues in Human Genetics

Three credits. Prerequisite: Open to junior and senior Allied Health Sciences and Diagnostic Genetic Sciences majors, others with consent of instructor.

Historical and contemporary issues relevant to human genetics, including the layperson’s understanding of genetic testing and diagnosis; and the ethical, legal, and social issues associated with them.

4248. Advanced Karyotyping and Report Writing

Two credits. Two hours discussion; 6-8 hours laboratory by arrangement. Prerequisites: DGS 3222, 3223 and DGS 4224; open only to students enrolled in the Diagnostic Genetic Sciences Program.

Karyotyping constitutional and cancer cytogenetic cases, diagnosing cytogenetic syndrome/conditions and cytogenetic changes associated with neoplasms. Writing complete, precise, and accurate cytogenetic reports.

4501. Specimen Processing

Two credits. Prerequisite: In order to enroll in this course, a student must have earned a “C” or better in DGS 4234 and 4235; open to DGS molecular students only.

Theory and methods for processing and evaluating specimens for nucleic acid isolation.

4502. Nucleic Acid Isolation

Four credits. Prerequisite: In order to enroll in this course, a student must have earned a “C” or better in DGS 4234 and 4235; open to DGS molecular students only.

Isolation, manipulation and evaluation of nucleic acids.

4503. Amplification Methods

Six credits. Prerequisite: In order to enroll in this course, a student must have earned a “C” or better in DGS 4234 and 4235 and 4236; open to DGS molecular students only.

Practicum experience in DNA and/or RNA amplification stressing polymerase chain reaction.

4510. In Situ Hybridization Methods

Two credits. Prerequisite: In order to enroll in this course, a student must have earned a “C” or better in DGS 4234 and 4235; open to DGS molecular students only.

Practicum in fluorescence in situ hybridization or other in situ hybridization techniques.

4512. Cloning Techniques

Two credits. Prerequisite: In order to enroll in this course, a student must have earned a “C” or better in DGS 4234 and 4235; open to DGS molecular students only.

Theory and techniques of cloning.

4513. Blotting Applications

Two credits. Prerequisite: In order to enroll in this course, a student must have earned a “C” or better in DGS 4234 and 4235; open to DGS molecular students only.

Theory and techniques of nucleic acid and/or protein blotting (e.g. Southern blot, reverse clot blot).

4514. DNA Sequencing

Two credits. Prerequisite: In order to enroll in this course, a student must have earned a “C” or better in DGS 4234 and 4235; open to DGS molecular students only.

Practicum experience in DNA sequencing.

4515. Microbiological Applications of Molecular Diagnostics

Two credits. Prerequisite: In order to enroll in this course, a student must have earned a “C” or better in DGS 4234 and 4235; open only to students enrolled in the Molecular Diagnostic Sciences Program; others with consent of the instructor.

Practicum experience in the application of molecular technologies to microbiology.

4810. Suspension Cell Culture, Harvest, and Analysis

Six credits. Practicum course. Prerequisites: A grade of C or better in DGS 3222, 3223, 4224, and 4248; open only to DGS majors.

Techniques for processing suspension cell cultures and for isolating and identifying human chromosomes from suspension cell samples (bloods, bone marrows, and/or solid tumors). Culture, harvest, staining, microscopic analysis, and karyotyping of suspension cell samples.

4820. Attached Cell Culture, Harvest, and Analysis

Six credits. Practicum course. Prerequisites: A grade of C or better in DGS 3222, 3223, 4224, and 4248; open only to DGS majors.

Techniques for processing attached cell cultures and for isolating and identifying human chromosomes from attached cell samples (amniotic fluids, chorionic villus samples, products of conception, skin biopsies, and/or tumors, etc.). Culture, harvest, staining, microscopic analysis, and karyotyping of attached cell samples.

4830. Molecular Cytogenetic Technologies

Three credits. Practicum course. Prerequisites: A grade of C or better in DGS 3222, 3223, 4224, 4234/W, 4235, and 4248; open only to DGS majors.

Hands-on experience in fluorescence in situ hybridization (FISH) and other molecular cytogenetic technologies as applicable (e.g.: microarrays).

4850. Investigative Topics in Laboratory Genetics

One credit. Practicum course. Prerequisites: A grade of C or better in DGS 3222, 3223, 4224, 4234/W, 4235, 4236, and 4248; and AH 4241; open only to DGS majors.

Exploration of an area of individual interest in laboratory or clinical genetics.

4997. Honors Research

Three credits. Prerequisite: Open only to Diagnostic Genetic Sciences Honors students.

Design and implementation of an honors research project.

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